Production and Characterization of Protease from Serratia marcescens

Purification and Characterization of 50 kDa Extracellular Metalloprotease from Serratia sp. ZF03

Background: Proteolytic enzymes have an important role in variety of physiological and pathological functions. They have been used in therapeutic and pharmaceutical applications. Characterizations of extracellular proteases from various strains of S. marcescens indicate that most strains produce a very similar major metalloprotease. This metalloprotease (serrapeptidase, serrapeptase) is an impo...

Serratia marcescens B4A Chitinase Product Optimization Using Taguchi Approach

Chitinase production by newly isolated Serratia marcescens B4A was optimized following Taguchi’sarray methods. Twenty-three bacterial isolates were screened from shrimp culture ponds in the South ofIran. A chitinase-producing bacterium was isolated based on it’s ability to utilize chitin as the sole carbonsource. The isolate designated as B4A, was identified as Serratia marces...

Production of L-Asparaginase by Serratia marcescens SB08: Optimization by Response Surface Methodology

This paper describes optimization method that combines the Plackett-Burman design, a factorial design and the response surface method, which were used to optimize the medium for the production of L-asparaginase by Serratia marcescens SB08. Four medium factors, from out of 11 medium factors, were screened by Plackett-Burman design experiments and subsequent optimization process to find out the o...

A Novel Production and Characterization of Serratiopeptidase from Serratia Marcescens

Application of Response Surface Methodology in Medium Optimization for Protease Production by the New Strain of Serratia marcescens SB08 CHIDAMBARAM KULANDAISAMY VENIL* and PERUMALSAMY LAKSHMANAPERUMALSAMY

For production of protease by a new strain, Serratia marcescens SB08, optimization of the fermentation medium and environmental conditions, were carried out by applying factorial design and response surface methodology. The results of factorial design showed that pH, agitation, incubation time and yeast extract were the key factors affecting protease production. The optimal cultural conditions ...